Structure and Biosynthesis of the Antibiotic Bottromycin D
نویسندگان
چکیده
Drug resistant infectious diseases are quickly becoming a global health crisis. While Streptomyces spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 Streptomyes spp. Using this methodology, we discovered bottromycin D and used whole genome sequencing to determine its biosynthesis by a ribosomal pathway.
منابع مشابه
Draft Genome Sequence of Streptomyces bottropensis ATCC 25435, a Bottromycin-Producing Actinomycete
A series of bottromycin antibiotics have been isolated and identified from Streptomyces bottropensis strain ATCC 25435. Here, a draft genome sequence of S. bottropensis ATCC 25435 is presented. The genome carries an intact biosynthetic gene cluster for bottromycin antibiotics, which provides insight into the combinatorial biosynthesis of bottromycin antibiotics.
متن کاملDissecting Bottromycin Biosynthesis Using Comparative Untargeted Metabolomics
Bottromycin A2 is a structurally unique ribosomally synthesized and post-translationally modified peptide (RiPP) that possesses potent antibacterial activity towards multidrug-resistant bacteria. The structural novelty of bottromycin stems from its unprecedented macrocyclic amidine and rare β-methylated amino acid residues. The N-terminus of a precursor peptide (BtmD) is converted into bottromy...
متن کاملErythromycin-inducible resistance in Staphylococcus aureus: survey of antibiotic classes involved.
Certain erythromycin-resistant strains of Staphylococcus aureus remain sensitive to other macrolide antibiotics. If these strains are exposed to low levels of erythromycin, resistance to other antibiotics is induced. The antibiotics to which resistance is induced by erythromycin include: other macrolides as well as lincosaminide, streptogramin (group B) antibiotics but not chloramphenicol, amic...
متن کاملSynergism between efrotomycin and bottromycin.
BETTINA M. FROST, MARY E. VALIANT and EUGENE L. DULANEY Merck Institute for Therapeutic Research Rahway, New Jersey, 07065, U.S.A. (Received for publication May 26, 1979) Efrotomycin1) is a narrow spectrum antibiotic which is most active in vitro against representative species of Pasteurella, Moraxella and Corynebacterium2) . The in vivo activity, however, is much better than one would expect f...
متن کاملMechanism of protein synthesis inhibition by bottromycin A2: studies with puromycin.
Bottromycin A2 inhibits polylysine synthesis with poly A at low concentrations of E. coli ribosomes. Peptide release by puromycin from ribosomes was significantly inhibited by blasticidin S, chloramphenicol and thiophenicol, but not by bottromycin A2 in the absence of GTPand G factor. Puromycindependent release of peptide from ribosomes was stimulated by the presence of GTP and G factor; it was...
متن کامل